3 Publications

3.1 Papers

3.1.6 Chronological list

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3.1.6.44 Yu, J., Abagyan, R., Dong, S., Gilbert, A., Nusenzweig, V., and Tomlinson, S. (1997). Mapping of the Active Site of CD59. J. Expt. Medicine, 185, 745-754

CD59 is a widely distributed membrane-bound inhibitor of the cytolic membrane attack complex (MAC) of complement. This small (77 amino acid) glycopeptide is a member of the Ly6 superfamily of proteins and is important in protecting host cells from the lytic and proinflammatory activity of the MAC. CD59 functions by binding to C8 and/or C9 in the nascent MAC and interfering with C9 membranne insertion and polymerization. We present data obtained from a combination of molecular modeling and mutagenesis techniques, which together indicate that the active site of CD59 is located in the vicinity of a hydrophobic groove on the face of the molecule opposite to a "hydrophobic strip" suggestied earlier. In addition, removal of the single N-linked glycosylation site at Asn18 of CD59 resulted in an enhancement of compliment inhibitory activity.